Identification of an autonomously replicating sequence of the flavinogenic yeast Pichia guilliermondii
نویسندگان
چکیده
منابع مشابه
A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production
Pichia pastoris is a non-conventional methylotrophic yeast that is widely used for recombinant protein production, typically by stably integrating the target gene into the genome as part of an expression cassette. However, the comparatively high clonal variability associated with this approach usually necessitates a time intense screening step in order to find strains with the desired productiv...
متن کاملMechanism of Gene Amplification via Yeast Autonomously Replicating Sequences
The present investigation was aimed at understanding the molecular mechanism of gene amplification. Interplay of fragile sites in promoting gene amplification was also elucidated. The amplification promoting sequences were chosen from the Saccharomyces cerevisiae ARS, 5S rRNA regions of Plantago ovata and P. lagopus, proposed sites of replication pausing at Ste20 gene locus of S. cerevisiae, an...
متن کاملIdentification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe.
Autonomously replicating sequence (ARS) elements of the fission yeast Schizosaccharomyces pombe contain multiple imperfect copies of the consensus sequence reported by Maundrell et al. [Maundrell K., Hutchison, A. & Shall, S. (1988) EMBO J. 7, 2203-2209]. When cell free extracts of S. pombe were incubated with a dimer or tetramer of an oligonucleotide containing the ARS consensus sequence, seve...
متن کاملMolecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was vali...
متن کاملValidation of the species Pichia guilliermondii.
Many species of yeasts exist in nature in haploid form. If strongly heterothallic, strains of such species produce no ascospores when tested individually. They do mate if sexually reactive strains of opposite sex are mixed on a sporulation medium. In 1952, L. J. Wickerham and K. A. Burton (J. Bacteriol. 63:449, 1952) reported that all strains of an imperfect form known variously as Dematium cho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 2000
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.000555